New Component Tools for Synthetic Biology:A highly photostable and bright green fluorescent protein
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- Time of issue:2022-05-06 16:32
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New Component Tools for Synthetic Biology:A highly photostable and bright green fluorescent protein
(Summary description)In the field of synthetic biology, fluorescent proteins are common component tools. However, obtaining high signal-to-noise-ratio images from samples with low-level expression requires strong excitation light, which often causes severe photobleaching.
- Categories:News
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- Time of issue:2022-05-06 16:32
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In the field of synthetic biology, fluorescent proteins are common component tools. However, obtaining high signal-to-noise-ratio images from samples with low-level expression requires strong excitation light, which often causes severe photobleaching. Similarly, obtaining a high spatiotemporal resolution to study the fast dynamics of fine subcellular structures requires continuous acquisition of images on a time scale from seconds to minutes, which inevitably leads to noticeable FP photobleaching. Highly photostable FPs are, therefore, needed to enable fast and long super-resolution imaging.
Recently, the researchers modified the wild-type green fluorescent protein GFP found in the jellyfish Cytaeis uchidae to obtain a highly photostable and bright green fluorescent protein StayGold (GenBank accession number: LC601652).StayGold is over one order of magnitude more photostable than any currently available fluorescent protein and has a cellular brightness similar to mNeonGreen. StayGold will substantially reduce the limitations imposed by photobleaching, especially in live cell or volumetric imaging.
The current fluorescent protein, StayGold, exists as a dimer, and the properties of the dimer may limit the use of this fluorescent protein for membrane protein labeling and protein fusion. A monomeric version of StayGold (mStayGold) is under development. The mStayGold will then enable the visualization and quantification of tagged proteins at low copy number expressed via genome editing techniques; it will also enable the tracking of such proteins at the single-molecule level in cells over extended periods of time.
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